ELISA, Western Blot. This antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 110 kDa in size corresponding to MTBP by western blotting in the appropriate cell lysate or extract.
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Description
This antibody is directed against human MTBP protein. The product was produced from clarified mouse ascetic fluid. A BLAST analysis was used to suggest reactivity with this protein from human (100% homology) and chimpanzee. Cross reactivity with MTBP protein from mouse (77% homology) and chicken (79% homology) is unlikely due to the relatively low number of positive residues and high number of gaps within this sequence. Cross reactivity with MTBP homologues from other sources has not been determined.
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant amino-terminal HIS6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein.
Other Names
mouse anti-MTBP antibody, mouse anti-mouse double minute 2 antibody, MDM 2 BP antibody, MDM2 (mouse double minute 2) binding protein 104kD antibody, MDM2 (mouse double minute 2) binding protein antibody, MDM2 Binding Protein antibody, MDM2 BP antibody