ELISA, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to SMAD4 protein by western blotting in the appropriate cell lysate or extract.
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Description
This affinity purified antibody is directed against human SMAD4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with SMAD4 protein from human, mouse and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human SMAD4 protein.
Other Names
rabbit anti-SMAD4 antibody, SMAD-4, SMAD 4, mothers against decapentaplegic homolog 4 antibody, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4 antibody, SMAD family member 4, DPC 4, DPC4, MADH4, MADH 4