ELISA, IF Microscopy, Western Blot. Protein G Purified Mab anti-SMC1 was tested by ELISA, immunohistochemistry and western blotting against native protein. The antibody reacts with SMC1 from irradiated human and mouse cells. A 160 kDa band corresponding to human SMC1 is noted in
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Description
This Protein G Purified Mab antibody is directed against human SMC1 and is useful in determining its presence in various assays. This monoclonal anti-SMC1 antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. Minimal reactivity is observed against the non-phosphorylated epitope. Reactivity is observed against human and mouse SMC1. Cross reactivity with SMC1 from other eukaryotic sources has not been tested.
Immunogen
This antibody was produced from a synthetic peptide corresponding to aa 951-962 of human SMC1 by injection into a balb/c mouse.
Other Names
mouse anti-SMC1 pS957 antibody, Structural maintenance of chromosomes protein 1B antibody, SMC1beta protein antibody, SMC1B antibody, SMC1L2 antibody
Kitagawa, R., Bakkenist, C.J., McKinnon, P.J. and Kastan, M.B. (2004) Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. 18(12):1423-1438.Callen E, Jankovic M, Wong N, Zha S, Chen HT, Difilippantonio S, Di Virgilio M, Heidkamp G, Alt FW, Nussenzweig A, Nussenzweig M. (2009) Essential Role for DNA-PKcs in DNA Double-Strand Break Repair and Apoptosis in ATM-Deficient Lymphocytes. Mol Cell. 34(3):285-97.