FLISA, Flow Cytometry, IF Microscopy. F(ab')2 Anti-Human IgM Fc5μ Texas Red Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG or Human IgA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.