ELISA, Immunohistochemistry, Western Blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:190,000 is suggested for this product.
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Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgM and Human Serum. No reaction was observed against Human IgG or Human IgA.
Immunogen
Human IgM Fc5μ fragment
Other Names
Rabbit Anti-Human IgM (Fc5u) Antibody Biotin Conjugated, Rabbit Anti Human IgM Fc5u Antibody Biotin Conjugated