Flow Cytometry, IF Microscopy. F(ab')2 Anti-Human IgM Fc5μ Phycoerythrin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
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Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Human IgM, Human IgM Fc5μ and Human Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine or Horse Serum Proteins, or other human heavy or light chain proteins.