ELISA, Immunohistochemistry, Western Blot. Anti-TNF alpha antibody has been tested in WB, ELISA, and IHC. Expect a band in appropriate lysates at ~25.6kDa. This antibody is also useful for neutralization of human and primate TNFα activity in bioassays diluted at 1:200. The antibody does not recognize human TNF beta (lymphotoxin). Specific conditions for reactivity and signal detection should be optimized by the end user.
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Description
This antibody is primarily directed against mature 17,000 MW human TNFα and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFα in the same formats using similar dilutions. The antibody does not recognize human TNFβ (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFα as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFα activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Immunogen
TNF alpha antibody used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFα.