ELISA, IF Microscopy, Western Blot. This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract.
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Description
This affinity purified antibody is directed against human MAD2L1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, dog, macaque, chimpanzee and gecko based on 100% homology for the immunogen sequence. Cross reactivity with MAD2L1 may occur from mouse and chicken sources, as only a two amino acid residue change is found within the immunogen sequence (90% positive by BLAST). Cross reactivity with MAD2L1 homologues from other sources has not been determined.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 3-13 of Human MAD2L1 protein.
Other Names
rabbit anti-MAD2L1 antibody, MAD2, MAD2-like protein 1, HsMAD2, Mitotic arrest deficient 2-like protein 1, Mitotic spindle assembly checkpoint protein MAD2A