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| Antigenic Specificity | NF-Y, N-terminus |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human |
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| Isotype | n/a |
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| Format | ion exchange purified |
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| Size | 100 μg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Western Blot. This product was assayed by immunoblot and found to be reactive against the 25 kDa B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (B subunit specific). Minimal reaction was observed by immunoblot against the 35 kDa A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 μl per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using (#611-1302) Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS substrate (#ABTS-100). A dilution of 1:4000 is suggested from this experiment. Minimal reactivity was observed by ELISA against the A subunit of NF-Y. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This product was prepared from monospecific antiserum by a multi-step procedure which includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis. |
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| Immunogen | NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
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| Other Names | rabbit anti-NF-Y Antibody, Nuclear transcription factor Y subunit B |
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| Gene, Accession # | NFYB, Gene ID: 4801, NCBI: P25208.2, UniProt: P25208 |
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| Catalog # | 200-401-101 |
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| Price | |
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| Order / More Info | NF-Y, N-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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