ELISA, Gel Shift, Immunohistochemistry, Western Blot. Anti-NF-Y (A subunit) Antibody was assayed by immunoblot and found to be reactive against NF-Y (A subunit specific) at a dilution of 1:6,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-NF-Y (A subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y. Negligible reactivity was observed against the B subunit of NF-Y. Anti-NF-Y (A subunit) Antibody was also tested in a gel supershift assay and found to be reactive against NF-Y using 1.0 to 2.0 μl per assay. Specific conditions should be optimized by user.
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Description
Anti-NF-Y (A subunit) Antibody was prepared from monospecific antiserum by delipidation and defibrination.
Immunogen
NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Other Names
rabbit anti-NF-Y antibody, Nuclear transcription factor Y subunit alpha, Nuclear transcription factor Y subunit A, NF-YA, CAAT box DNA-binding protein subunit A