ELISA, Immunohistochemistry, Western Blot. This antibody has been tested for use in ELISA and western blotting. This antibody is specific for HIF-1a hydroxylated at P564. Minimal cross-reactivity occurs with non-hydroxylated HIF-1a. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 110 kDa in size corresponding to HIF-1a hydroxyP564 by western blotting in the appropriate cell lysate or extract.
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Description
This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus sources based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1a.
Other Names
rabbit anti-Hif-1 alpha hydroxy P564 Antibody, Hypoxia-inducible factor 1-alpha antibody, ARNT-interacting protein antibody, Member of PAS protein 1 antibody, Basic-helix-loop-helix-PAS protein MOP1 antibody, PAS domain-containing protein 8 antibody, Class E basic helix-loop-helix protein 78 antibody