human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
Isotype
n/a
Format
immunoaffinity purified
Size
50 μg
Concentration
0.96 mg/ml
Applications
Chip, Immunohistochemistry, IF Microscopy, Western Blot. Anti-Histone H3 [p Ser28] antibody is useful for Western Blot, Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus? antibody production in collaboration with Novus Biologicals.
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Description
Anti-Histone H3 [p Ser28] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Immunogen
Histone H3 [p Ser28] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Serine 28 of human Histone H3.