human, chimpanzee, orangutan, mouse, rat, chicken, dog
Isotype
n/a
Format
immunoaffinity purified
Size
100 μg
Concentration
1.7 mg/mL
Applications
ELISA, Immunoprecipitation, Western Blot. This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 104-107 kDa in size corresponding to AP2A1 or AP2A2 proteins by western blotting in the appropriate cell lysate or extract. Multiple isoforms of each subunit exist for these proteins. The immunogen sequence is found in both AP2A1 and AP2A2. This antibody recognizes a band of approximately 100 kDa in multiple human cell lines and specific reactivity can be blocked with the immunizing peptide.
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Description
This affinity purified antibody is directed against human AP2A. The product was affinity purified from antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with AP2A1 and AP2A2 protein from human, chimpanzee, orangutan, mouse, rat, chicken and dog based on 100% homology for the immunogen sequence. Cross reactivity with AP2A proteins from other sources is not known.
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 3-14 of Human AP2A1 and AP2A2 proteins.
Other Names
goat anti-AP2A Antibody, AP-2 complex subunit alpha-1, Adapter-related protein complex 2 alpha-1 subunit, Adaptor protein complex AP-2 subunit alpha-1 Alpha1-adaptin, Plasma membrane adaptor HA2/AP2, Clathrin assembly protein complex 2 alpha-A large chain, AP2A2, ADTAB, CLAPA2, HIP9, HYPJ, KIAA0899