ELISA, Immunohistochemistry, Western Blot. This antibody reacts with human APC11 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins (APC2) does occur. A 9.8 kDa band corresponding to human APC11 is detected. Most cell lines or tissues expressing APC11 can be used as a positive control. Researchers should determine optimal titers for other applications.
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Description
This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse APC11. Cross reactivity may also occur with APC11 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 76-84 of Human APC11 (C-terminal) coupled to KLH.
Ohta T,Michel JJ,Xiong Y, (1999) Association with cullin partners protects ROC proteins from proteasome-dependent degradation. Oncogene 18:48, 6758-6766.Furukawa M,Ohta T,Xiong Y, (2002) Activation of UBC5 ubiquitin-conjugating enzyme by the RING finger of ROC1 and assembly of active ubiquitin ligases by all cullins. J Biol Chem 277:18, 15758-15765.