ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-Apolipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, and for western blotting for highly sensitive qualitative analysis. When performing immunoblotting assays - do not block with blotto. Blotto formulations tend to contain apoLipoprotein Type C-III and will neutralize antibody activity.
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Description
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Immunogen
produced from apoLipoprotein Type C-III derived from human plasma
Other Names
goat anti-Apolipoprotein C-III antibody, ApoC-III, Apo-CIII antibody, Apolipoprotein C3, Apolipoprotein C III antibody