Western Blot. Anti-Human Uromucoid Antibody has been tested in IEP, WB, and radial immunodiffusion (RID). May be useful in multiple assays. Specific conditions for reactivity should be optimized by the end user.
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Description
This polyclonal antibody is directed against Human Uromucoid and is an IgG fraction purification by precipitation and anion exchange chromatography. Cross reactivity to other species has not been tested. Lot variation tested and limited to ±20% by radial Immunodiffusion (RID). Tamm-Horsfall glycoprotein (THP), also known as uromodulin, is a glycoprotein that in humans is encoded by the UMOD gene. Uromodulin is the most abundant protein excreted in ordinary urine. The excretion in urine follows proteolytic cleavage of the ectodomain of its glycophosphatidylinositol-anchored (GPI-anchored) counterpart that is situated on the luminal cell surface of the loop of Henle. The excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria and may act as a constitutive inhibitor of calcium crystallization in renal fluids. Uropontin, nephrocalcin and uromodulin are the three known urinary glycoproteins that affect the formation of calcium-containing kidney stones or calculus. Tamm-Horsfall protein is part of the matrix in renal calculi but a role in kidney stone formation remains debatable. However, decreased levels of Tamm-Horsfall in urine have been found to be a good indicator of kidney stones. Anti-Human Uromucoid (Tamm-Horsfall glycoprotein) Antibody is useful for researchers interested in cancer research and Glucose Energy Metabolism.
Immunogen
Anti-Human Uromucoid was produced in sheep by repeated immunizations with human uromucoid, purified from human urine, shown to be greater than 95% pure by SDS-PAGE.