Bovine IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Bovine IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum and anti-Bovine IgM (μ chain specific). No reaction was observed against anti-Bovine IgG F(c). Some light chain cross reactivity will occur with anti-Bovine IgG.
Size
1 mg
Concentration
1.0 mg/mL
Applications
ELISA, Immunohistochemistry, Western Blot. Bovine IgM whole molecule can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other Names
Cow immunoglobulin M. IgM whole molecule, Bovine IgM