VlsE is a fusion protein with an MBP tag and was expressed in E.coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified VlsE and was estimated to be greater than 90% pure.
Size
100 μg
Concentration
1.0 mg/mL
Applications
ELISA, Western Blot. VlsE is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 78.7 kDa for VlsE-MBP, (36.3 kDa for VlsE and 42.4 kDa for MBP) in size corresponding to VlsE by Western blotting in the appropriate cell lysate or extract.
Other Names
Outer surface protein VlsE, Borrelia burgdorferi VlsE, vlsE protein, control protein