Non-viable cell pellet was generated from cell line WM1361A. WM1361A is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. These cells display mesenchymal morphology in culture. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. This cell line also expresses PTEN loss of function including hemizygous PTEN deletion and is wild type for BRAF, c-KIT, and CDK4. WM1361A cells produce xenograft tumors when injected into immunocompromised mice.
Size
1 each
Concentration
n/a
Applications
Frozen non-viable cell pellets can be used for in vitro experiments such as Western blotting and other immunoassays, genomic DNA isolation, STR profiling, and RNA isolation.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft