Total RNA was prepared from cell line WM293A. WM293A is a metastatic human melanoma cell line with thin elongated fibroblastic morphology. This cell line was derived from the same patient as the cell lines WM115, WM266-4, and WM165-1. WM115 cell line originated from the primary tumor, and WM293A, WM165-1 and WM266-4 were from individual lymph-node metastases. The subject (55-year-old female) displayed VGP with a Clark level III tumor with thickness of 2.24mm.This cell line features the specific V600D (Val600Asp) mutation at codon 600 in the BRAF gene. This cell line also expresses PTEN loss of function including hemizygous deletion. WM293A cell line is wild type for N-RAS, c-KIT, and CDK4 genes. WM293A cells produce xenograft tumors when injected into immunocompromised mice.
Size
10 μg
Concentration
200 ng / μl
Applications
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiements.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft