Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Light and heavy chain composition has been confirmed.
Size
1 mg
Concentration
1.0 mg/mL
Applications
ELISA, FLISA, Flow Cytometry, IF Microscopy. Mouse IgG1 isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.