Recombinant full-length human 4EBP1 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.
Size
20 μg
Concentration
0.2 μg/μL
Applications
Western Blot. 4EBP1 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. 4EBP1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 19kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Other Names
BP-1, EIF4EBP1, PHAS-I, MGC4316, Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1