Recombinant full length Xenopus UBE2D2 (UBC4) was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
Size
20 μg
Concentration
0.1 μg/μL
Applications
Western Blot. UBE2D2 Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. UBE2D2 Protein is suitable for use in Western Blot. Expect a band approximately ~17kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.