Recombinant human Ubiquitin (amino acids 1-76) protein was expressed by E. coli cells using an N-terminal his epitope. The purity was determined to be >95% by densitometry.
Size
20 μg
Concentration
0.2 μg/μl
Applications
Western Blot. Ubiquitin Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol. Ubiquitin Protein is suitable for use in Western Blot. Expect a band approximately ~10kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.