Product Specifications: Rat IGFBP-4: Rat IGF Binding Protein-4 (rIGFBP-4) is a 233 amino acid glycosylated protein that is one of the six 'classical' IGF binding proteins. These binding proteins have an important role in stabilizing and regulating the activity of IGFs in vivo. Rat IGFBP-4 binds both IGF-I and IGF-II and modifies their biological activity and bioavailability. Link to Sequence.
Spencer, E.M. et al. (1999) Proc. Endocrine Soc. (US) p405 (P2-588) Chernausek, S.D. et al. (1995) J. Biol. Chem, 270, 11377-11382. Source: Produced recombinantly in E. coli. Purified by affinity and other chromatographic steps. Purity:> 95% rat IGFBP-4 (as determined by MassSpectrometry) Molecular weight:25.7 kDa (non-glycosylated) (confirmed by Mass Spectrometry) Quantity:15 μg Appearance: Lyophilized white powder Biological Activity: Binding of 125I-IGF-I: IC50 2.4 x 10-1 nM State:Dried from 10 mM HCl Reconstitution:Reconstitute in 75 μl 10 mM HCl at a concentration of 0.2 mg/ml. Aliquot, snap freeze and store at -80°C. Avoid freeze- thaw cycles.Storage/Stability: At least 2 years at 2-4°C (lyophilized) This a Novozymes GroPep Specification! ibt is authorized distributor of GroPep!