Sample Type: Serum, plasma, tissue homogenates and other biological fluids. Intended Use: For quantitative detection of 15-LO/LOX in serum, plasma, tissue homogenates and other biological fluids. Sensitivity: <0.094ng/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Range: 0.156-10ng/ml Specificity: Mouse Test Principle: This BioAssay? kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-15-LO/LOX antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-15-LO/LOX antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to