Myeloperoxidase (MPO: EC 1.11.2.2) is a peroxidase abundantly expressed in neutrophil granulocytes. It catalyzes the hydrogen peroxide (H2O2) dependent oxidation of chloride anions (Cl-) to generate hypochlorite (HClO). During respiratory burst, neutrophils utilize MPO activity to exhibit cytotoxicity against tumor cells and pathogenic microorganisms. MPO also oxidizes various substances such as phenols and anilines, which can lead to inflammation. Increased MPO levels are associated with an increased risk of cardiovascular disease and myocardial infarction. Thus measurement of MPO peroxidation activity is crucial for disease prediction and therapeutics. The Myeloperoxidase (MPO) Peroxidation Activity Assay Kit, oxidizes a substrate to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to total peroxidase activity in the sample. A specific MPO inhibitor is provided, which suppresses peroxidase activity due to MPO. This permits differentiation of MPO-mediated peroxidation from the activity of other peroxidases which may be present in the samples. Our kit provides a sensitive, quick and easy method for detecting MPO activity. The assay is high-throughput adaptable and can detect less than 2 μU of MPO activity. Sample types: adherent cells, animal tissues, heart, liver, spleen, suspension cells
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Applications
Measurement of MPO activity in various tissues/cells.Screening MPO inhibitors for drug development