ELISA, Western Blot. Anti-ATDC affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity and detection of ATDC should be optimized by the end user. Expect a band approximately ~66 kDa in size corresponding to ATDC by western blotting in the appropriate cell lysate or extract.
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Description
Anti-ATDC antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with over-expressed, acetylated and non-acetylated (at K116) ATDC protein. A BLAST analysis was used to suggest cross-reactivity with ATDC from human, horse, bovine, chimpanzee and macaque based on a 100% homology with the immunizing sequence. Partial reactivity is expected against rat and mouse ATDC based on 92% homology with the immunizing sequence. Cross-reactivity with ATDC from other sources has not been determined.
Immunogen
Anti-ATDC affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.
Other Names
rabbit anti-ATDC Antibody, Ataxia-telangiectasia group D associated protein antibody, FLJ36085 antibody, TRIM 29 antibody, Tripartite motif containing protein 29 antibody, TRIM-29