Influenza A H5N1 (Avian Flu) Hemagglutinin / HA ELISA Pair Set
Description
The H5N1 (avian flu) hemagglutinin ELISA Pair Set is for the quantitative determination of hemagglutinin.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for hemagglutinin coated on a 96-well plate. Standards and samples are added to the wells, and any hemagglutinin present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-hemagglutinin polyclonal antibody is then added, producing an antibody-antigen-antibody sandwich. To produces color in proportion to the amount of hemagglutinin present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.