Immunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response.{20137,20136,20138,20141,20140,20139} OVA/alum immunized mice produce anti-OVA antibodies predominantly of the IgG1 and IgE isotypes that mediate tissue-specific effector functions in multiple mouse models of chronic inflammation, including allergic asthma, allergic rhinitis, and cutaneous hypersensitivity.{20137,20136,20138,20141},When using one of these models, it is often desirable to measure anti-OVA antibody levels in the plasma or serum to determine the effectiveness of the immunization, the activity of a drug, or the effect of a specific gene deletion. IgG1 is the predominant anti-OVA immunoglobulin isotype found in the serum or plasma of mice immunized with OVA/alum; the plasma concentration of OVA-specific IgG1 is typically 1,000-fold greater than that of OVA-specific IgE.{20137} Therefore, the measurement of anti-OVA IgG1 is a commonly used method of assessing the magnitude of this TH2 immune response.,Cayman's Anti-Ovalbumin IgG1 (mouse) ELISA Kit is an immunometric (i.e., ``sandwich'') assay which can be used to measure anti-ovalbumin of the IgG1 isotype in mouse plasma and serum without prior sample purification. Affinity-purified anti-ovalbumin IgG1 isolated from the plasma of mice immunized with OVA/alum is used as the standard. The standard curve spans the range of 1.56-200 ng/ml, with an LLOQ of 1.56 ng/ml.