Purity: > 90% by SDS - PAGE Format: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF. NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. Recombinant human NEIL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.