Protein Expression Media, Superior Broth? (Liquid) pEX?
Description
Superior Broth? and Superior Prime Broth? are proprietary medium formulations for improved expression of recombinant proteins in E. coli. Not sure which media expression system to choose? United States Biological has developed a unique research tool for protein expression media selection: Media Optimization Kit?: Superior Prime Broth? is made without animal-derived raw materials and is APF Certified. The media are complex, rich formulations which supply a nitrogen source, vitamins, and moderate glucose levels. The media are buffered at pH 7.2±0.2 to prevent acidification of the medium during metabolism of the glucose. Expression of Recombinant Proteins: For expression of recombinant proteins, induction should be done at cell densities twice that of LB Broth. Cell yields are typically about 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Plasmid copy numbers are the highest among the pEX? family of media. This medium is an exc