Protein Expression Media, Power Prime Broth? (Liquid) pEX?
Description
Power Broth? and Power Prime Broth? are proprietary media formulations for improved expression of recombinant proteins in E. coli. Power Prime Broth? is made without animal-derived raw materials and is APF Certified. These media are composed of a complex, rich mixture of amino acids, vitamins, and a carbon source at higher levels than Terrific Broth. The media are buffered at pH 6.8±0.2. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Several reports suggest that Power Broth? and Power Prime Broth? can increase the amount of soluble protein accumulated for otherwise insoluble proteins. This medium is suitable as the base for fermentation cultures. Biomass yields will depend on the process and the strain employed.