Sirtuin 1 is the human ortholog for the yeast Sir2 (silent information regulator 2) protein regulating epigenetic gene silencing as a possible antiaging effect. Sirtuin 1 is an NAD(+)-dependent histone deacetylase, which deacetylate lysines 9 and 14 of histone H3 and lysine-16 of histone H4, involved in various cellular functions such as transcription, energy sensing, and differentiation. Sirtuin1 plays an important role in regulating adipogenesis via repression of PPAR and the gluconeogenic/glycolytic pathways in liver in response to fasting signals through the transcriptional coactivator PGC1A deacetylated at specific lysine residues in an NAD(+)-dependent manner. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quan