Immunohistochemistry, Western Blot. Anti-Caspase 3 Antibody is suitable for use in IHC (frozen), IHC (paraffin), and WB. Expect a band approximately 31.5 kDa on specific lysates. The antibody detects both pro Caspase-3(32 kDa) and the large subunit of the active/cleaved form (14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. Specific conditions for reactivity should be optimized by the end user.
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Description
Anti-Caspase-3 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-3 from Human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Caspase-3 from other sources has not been determined.
Immunogen
Caspase-3 Antibody was produced in mice by repeated immunizations with a full length recombinant Caspase-3 human protein.