Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 0.1 M NaCl, 2mM DTT, and 20% Glycerol . Midkine, also known as Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor of low molecular weight, a member of the NEGF family whose founding member is pleiotrophin. This protein exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. Recombinant human Midkine protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.