Human Synaptic Vesicle Antibody Sampler Kit, BioAssay?
Description
Fusion of a vesicle to its target membrane is a universal process in eukaryotic cells for proper cellular organization and function. Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes. SNAP25 forms a core complex with the SNARE proteins syntaxin and synaptobrevin to mediate synaptic vesicle fusion with the plasma membrane during Ca2+-dependent exocytosis. Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins. Munc18-1 acts as a molecular chaperone for syntaxin-1, allowing for formation of the SNARE complex at the plasma membrane. Following membrane fusion, the alpha-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the alpha-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion. The ATPase activity of NSF i