Hyaluronic Acid (HA) BioAssay? ELISA Kit is a quantitative competitive assay for the detection of HA in rat serum, plasma, tissue homogenates and other biological fluids. Detection Range: 1.563-100ng/ml Sensitivity: <0.938ng/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Assay Principle: The microtiter plate provided in this kit has been pre-coated with HA. HA in the standard or sample competes with a fixed amount of plate-coated HA for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of HA in the samples is determined by an inverse correlation between HA in the sample an