Recombinant Human Fc gamma RIIIA/CD16a (carrier-free)
Description
Fcγ RIII, also known as CD16, is a low affinity IgG Fc receptor for a broad range of different isotypes. It primarily exists as two distinct forms: Fcγ RIIIA/ CD16a and Fcγ RIIIB/ CD16b, which are encoded by two nearly identical genes in humans. The expression of the two isoforms is tissue-specific. Fcγ RIIIA is a 50-65 kD type I transmembrane protein that is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. FcγRIIIB is a 48 kD GPI-anchored protein. Its extracellular domain is over 95% homologous to that of Fcγ RIIIA, and it is expressed specifically on neutrophils. Fcγ RIIIA can bind aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC). Several studies indicated that the post-translational modifications of Fcγ RIIIA or IgG regulate Fcγ RIIIA-mediated functions. The glycosylation of Fcγ RIIIA is mandatory for high affinity binding to Fc. Additionally, lack of fucosylation of Fc glycans dramatically enhance ADCC by improving the binding of Fcγ RIIIA and IgG. Is has been reported that the polymorphism (F176V) showed a greater binding capacity to IgG, leading to increasing antibody-dependent NK cell cytotoxicity and susceptibility to autoimmune diseases. The missense mutation (L66H) causes immunodeficiency 20 disease (IMD20). Fcγ RIIIA associates with γ and zeta chains of T-cell antigen receptor complex (CD3:TCR) and FcεRIγ on human NK cells, suggesting involvement of distinct signal-transducing function.
Size
25 μg
Concentration
n/a
Applications
BA
Other Names
Low affinity immunoglobulin gamma Fc region receptor III-A, CD16a antigen, CD16a, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2, FCGR3, FCGR3A, IGFR3