RNase Inhibitor, Mammalian specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 10^14.
Size
2000 U / 10000 U / 20000 U
Concentration
n/a
Applications
Cell-Free Protein Expression, PCR, RT-PCR,cDNA synthesis,In vitro transcription/translation,Enzymatic RNA labeling reaction,Other applications where the integrity of RNA is important