The Interleukin-17C (IL-17C) BioAssay? ELISA Kit (Mouse) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IL-17C in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. Detection Range: 15.6-1,000pg/ml Sensitivity: <5.8pg/ml Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-17C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IL-17C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL-17C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measur