Interleukin 1 Family, Member 9 (IL1F9) BioAssay? ECL Kit (Mouse) is a quantitative, in vitro, Chemiluminescence-based sandwich immunoassay (ECL) suitable for use in Serum, plasma and other biological fluids. Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Family, Member 9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Family, Member 9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 1 Family, Member 9 (IL1F9) level in the sample or standard. Detection Range: 15.625-1000pg/ml Sensitivity: The minimum detectable dose of this kit is typically less than 5.8pg/ml. Specificity: This sandwi