This IL-8 ELISA is a 2.5 hour solid phase immunoassay readily applicable to measure IL-8 levels in serum, plasma, cell culture supernatant and other biological fluids in the range of 50-1600pg/ml. It showed no crossreactivity with human IL-1b, EGF, MCAF, RANTES, and SAA. This IL-8 ELISA is expected to be effectively used for further investigations into the relationship between IL-8 and various diseases. Principle: This IL-8 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal specific for IL-8. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IL-8, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-8 and other components of sample. In order to quantitate the amount of IL-8 present in the sample, a standardize