Interleukin 10, Human (IL-10), BioAssay? ELISpot Kit
Description
Intended Use: This 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human IL-10. Principle: Add stimulated cells or cells and stimulant to the wells and incubate at 37°C in CO2 incubator for a specified period. Secreted human IL-10 binds to antibody coated ELISpot plate. ELISpot plate coated with human IL-10 Capture Antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbounded proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human IL-10. Background: The mature human IL-10 is a protein with 160 amino acids and the functional IL-10 is a homodimer. IL-10 is expressed by a variety of cells, including mouse T cells (TH1, TH2, and Tr1 subsets), B cells derived from peripheral blood, tonsils or spleen, Epstein-Barr virus (EBV)-transformed B cell lines, Burkitt's lymphoma, AIDS lymphomas, monocytes, placental troph