Interleukin 8, Human (IL-8), BioAssay? ELISpot Kit
Description
Intended Use: This 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human IL-8. Principle: Add stimulated cells or cells and stimulant to the wells and incubate at 37°C in CO2 incubator for a specified period. Secreted human IL-8 binds to antibody coated microtiter plate. microtiter plate coated with human IL-8 Capture Antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbounded proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human IL-8. Substrate Solution is added. A colored precipitate forms and appears as spots at the sites of human IL-8 secreting location. Each individual spot representing an individual human IL-8 secreting cell. Background: Interleukin-8 (IL-8), also known as neutrophil attractant/activating protein (NAP-1), monocyte-derived neutrophil-activating peptide (MONAP), monocyte-derived neu