Interleukin 6, Human (IL-6), BioAssay? ELISpot Kit
Description
Intended Use: This 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human IL-6. Principle: Add stimulated cells or cells and stimulant to the wells and incubate at 37°C in CO2 incubator for a specified period. Secreted human IL-6 binds to antibody coated microtiter plate. microtiter plate coated with human IL-6 Capture Antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbounded proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human IL-6. Substrate Solution is added. A colored precipitate forms and appears as spots at the sites of human IL-6 secreting location. Each individual spot representing an individual human IL-6 secreting cell. Background: Interleukin 6 (IL-6) is a multifunctional protein that plays important roles in host defense, acute phase reactions, immune responses, and hematopoiesis. (1) Syno