Creative Diagnostics employs three approaches to generate monoclonal anti-idiotype antibodies (anti-IDs). The first approach is to raise hybridoma clones in rats or mice. Any species of antibodies, whole IgG, scFv, Fab or F(ab')2, that preserve the antibody activity can be used for anti-IDs development. To enrich the antibodies that target the antigen-binding site, we usually cut the target antibody into Fab fragments, which could be used as immunogen (Antibody Fragmentation service). After screening against the immunogen, we can use isotype matching control antibodies and related immunoglobulin/proteins to do counter-selection. In this case, only the anti-IDs with the correct specificity are expanded. We further run corresponding assays to make sure the anti-IDs' lack of cross reactivity to non-specific antibodies and receptors. Additionally, our team can develop ELISA assays to support your studies, and set up neutralization and blocking assay to identify functional ligand blocking antibodies/receptors.More information, please visit the website: http://www.creative-diagnostics.com/Generation-of-monoclonal-anti-idiotype-antibodies.html