The Human Collagen Type IV (COL4) ELISA, ECL Kit is a sensitive chemiluminescent sandwich enzyme immunoassay for the in vitro quantitative measurement of COL4 in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 0.14-100ng/ml Sensitivity: <0.07ng/ml Precision: Intra-Assay CV: <10% Inter-Assay CV: <12% Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The addition of substrates A and B mixture generates glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the COL4 level in the sample or standard. Kit Components: *351750A: Microtiter Plate, 1x96 wells, Pre-coated with antibody; ready to use. *351750B: Stand