Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) BioAssay? ECL Kit (Canine) is a quantitative, in vitro, Chemiluminescence-based sandwich immunoassay (ECL) suitable for use in Serum, plasma and other biological fluids. Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) level in the sample or standard. Detection Range: 15.625-1000pg/ml Sensitivity: The minimum