Signal Transducer And Activator Of Transcription 3 (STAT3) BioAssay? ELISA Kit (Human, Mouse, Rat)
Description
The Signal Transducer And Activator Of Transcription 3 (STAT3) ELISA kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of STAT3 in human, mouse and rat tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. Detection Range: 1.56-100ng/ml Sensitivity: <0.61ng/ml Specificity: This assay has high sensitivity and excellent specificity for detection of STAT3. 100% cross-reactivity was observed among human, mouse and rat. Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to STAT3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to STAT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain STAT3, biotin-conjugated antibody and enzyme-con